Taurine Click to View Image C2H7NO3S 125.15
Taurine [107-35-7]. »Taurine contains not less than 98.5percent and not more than 101.5percent of C2H7NO3S,calculated on the dried basis. Packaging and storage— Preserve in well-closed containers. USP Reference standards á11ñ USP Taurine RS. Identification, Infrared Absorption á197Kñ. Loss on drying á731ñ Dry it at 105for 3hours:it loses not more than 0.3%of its weight. Residue on ignition á281ñ: not more than 0.3%. Chloride á221ñ A0.7-g portion shows no more chloride than corresponds to 0.50mLof 0.020Nhydrochloric acid.Not more than 0.05%is found. Sulfate á221ñ A0.8-g portion shows no more sulfate than corresponds to 0.25mLof 0.020Nsulfuric acid.Not more than 0.03%is found. Iron á241ñ: 0.003%. Heavy metals,Method Iá231ñ: 0.0015%. Chromatographic purity— Adsorbent: 0.25-mm layer of chromatographic silica gel mixture. Test solution— Dissolve an accurately weighed quantity of Taurine with water to obtain a solution having a concentration of about 10mg per mL. Standard solution— Dissolve an accurately weighed quantity of USP Taurine RSwith water to obtain a solution having a known concentration of about 0.05mg per mL,equivalent concentration to about 0.5%of the Test solution. Application volume: 5µL. Developing solvent system: a mixture of butyl alcohol,glacial acetic acid,and water (60:20:20). Spray reagent— Dissolve 0.2g ninhydrin in 100mLof a mixture of butyl alcohol and 2Nacetic acid (95:5). Procedure— Proceed as directed for Thin-Layer Chromatographyunder Chromatography á621ñ,except to dry the plate at 80for 30minutes.Spray the plate with Spray reagent,and heat at 80for about 10minutes.Examine the plate under white light:no secondary spot in the chromatogram of the Test solutionis larger or more intense than the principal spot in the chromatogram of the Standard solution.Not more than 0.5%of individual impurities are found.[NOTE—The RFvalue for the taurine spots should be about 0.2.] Organic volatile impurities,Method Iá467ñ: meets the requirements. Assay— Proceed as directed for Method IIunder Nitrogen Determination á461ñ.Each mLof 0.01Nsulfuric acid is equivalent to 1.25mg of C2H7NO3S. Auxiliary Information— Staff Liaison:Lawrence Evans,III,Ph.D.,Scientist Expert Committee:(DSN)Dietary Supplements:Non-Botanicals USP28–NF23Page 1849 Pharmacopeial Forum:Volume No.27(6)Page 3331 Phone Number:1-301-816-8389